[Autohemolysis of human erythrocytes in the temperature range 4-37 C]

It is shown that the value of activation energy of red blood cells autohemolysis in temperature range of 4 + 37 degrees C is considerably smaller than that at t > 37 degrees C and is about 50 kJ/mol in isotonic saline buffer, pH 7.4. This means that the change of limiting stage of hemolysis occurs at 37 degrees C. It is found that the decreasing of oxygen partial pressure in the medium resulted in marked inhibition of hemolysis without alteration of activation energy in temperature range of 20 divided by 37 degrees C and on the contrary in intensification of hemolysis at t < 20 degrees C. It is concluded that oxidative processes are essential in autohemolysis in temperature range of 20 divided by 37 degrees C and the oxygen stabilizes the structure state of erythrocytes below 20 degrees C.