Related Experiment Videos
FET-microbial sensor for xylose detection based on Gluconobacter oxydans cells
A N Reshetilov1, M V Donova, D V Dovbnya
1Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Puschino, Moscow Region, Russia.
Biosensors & Bioelectronics
|January 1, 1996
Summary
This study presents a novel biosensor for detecting xylose using whole cells of Gluconobacter oxydans and a field-effect transistor (FET). The developed biosensor demonstrates stability and sensitivity for accurate xylose quantification.
Area of Science:
- Biotechnology
- Biosensor Technology
- Enzyme Engineering
Background:
- Xylose is a key sugar with applications in various industries.
- Accurate and sensitive detection of xylose is crucial for quality control and research.
- Existing xylose detection methods may have limitations in terms of speed, cost, or specificity.
Purpose of the Study:
- To develop a novel potentiometric biosensor for xylose detection.
- To utilize Gluconobacter oxydans whole cells and a field-effect transistor (FET) for sensing.
- To evaluate the performance characteristics of the developed biosensor.
Main Methods:
- Whole cells of Gluconobacter oxydans were immobilized using physical adsorption.
- Extracellular pH changes due to xylose dehydrogenation were monitored using a FET.
- Sensor performance was assessed for sensitivity, linearity, stability, and interference.
Main Results:
- The biosensor detected xylose with a lower limit of 0.5 mM.
- A linear response was observed for xylose concentrations ranging from 5.0 to 30 mM.
- The sensor exhibited stability for over four weeks and minimal interference from xylitol, though glucose interference was noted.
Conclusions:
- A functional potentiometric biosensor for xylose has been successfully developed.
- The biosensor offers a sensitive and stable method for xylose quantification.
- A two-step assay is proposed to address interference from glucose in real-world samples.