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Quantitative analysis of pulmonary surfactant proteins B and C
M van Eijk1, C G De Haas, H P Haagsman
1Laboratory of Veterinary Biochemistry, Utrecht University, The Netherlands.
Analytical Biochemistry
|December 10, 1995
Abstract:
A procedure for the quantification of surfactant protein B (SP-B) and surfactant protein C (SP-C) in surfactant preparations is described. After butanol extraction of surfactant, the resulting extract is separated by Sephadex LH-60 HPLC, using an elution fluid which consists of 30% dichloromethane, 65% methanol, and 5% 100 mM HCL. SP-B and SP-C are detected by absorbance at 228 nm and quantified using standard stock solutions. Run length is approximately 70 min. Detection limits are 1 microgram SP-B and 4 micrograms SP-C.