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Updated: Aug 12, 2026
Isolation and Primary Culture of Mouse Aortic Endothelial Cells
Published on: December 19, 2016
Cloning and characterization of murine endothelial constitutive nitric oxide synthase
K Gnanapandithen1, Z Chen, C L Kau
1Department of Medicine, St. Michael's Hospital, University of Toronto, Canada.
Abstract:
Complementary DNA clones encoding mouse endothelial constitutive nitric oxide synthase (ecNOS) were isolated by plaque hybridization from a murine fetal cardiac lambda ZAP II cDNA expression library using a full-length human ecNOS cDNA as the hybridization probe. DNA sequence analysis indicates a 1202 amino acid protein showing significant sequence identity with human as well as bovine ecNOS.
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