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Serotyping of Cryptococcus neoformans by dot enzyme assay
T Belay1, R Cherniak, E B O'Neill
1Department of Chemistry, Georgia State University, Atlanta 30303, USA.
Journal of Clinical Microbiology
|February 1, 1996
Abstract:
A method is described for the serotyping of Cryptococcus neoformans based on direct analysis of culture supernatants for the major type-specific capsular antigen, glucuronoxylomannan. Factor sera prepared by absorption of polyclonal rabbit antisera (Iatron Laboratories, Inc., Tokyo, Japan) or selected anti-C. neoformans monoclonal antibodies were used in a dot enzyme assay to detect the presence of antigen.