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Observer variation in AgNOR counts in neoplastic breast lesions
A Srivastava1, P K Agarwal, M M Goel
1Department of Pathology, King George's Medical College, Lucknow, India.
Analytical and Quantitative Cytology and Histology
|June 1, 1996
Summary
Quantifying AgNOR (Argyrophilic Nucleolar Organizing Region) counts in breast lesions shows high observer variation. This variability limits the diagnostic and prognostic value of AgNOR quantitation in breast cancer.
Area of Science:
- Histopathology
- Oncology
- Biomarker Analysis
Background:
- Argyrophilic nucleolar organizing region (AgNOR) staining is a method used in histopathology.
- AgNORs are associated with cellular proliferation and have been investigated as potential biomarkers in various cancers, including breast lesions.
Purpose of the Study:
- To assess the reliability of AgNOR quantitation in breast lesions.
- To determine the extent of interobserver and intraobserver variability in AgNOR counting.
Main Methods:
- A standardized AgNOR counting method was applied to 45 breast lesions (20 benign, 25 malignant).
- Two independent observers performed counts within a defined area, with repeat counts after two weeks.
- Interobserver and intraobserver agreement was analyzed using the Bland-Altman method.
Main Results:
- Mean differences between observers and within observers were small.
- However, wide limits of agreement were observed, indicating substantial variability.
Conclusions:
- The observer variation in AgNOR counts is significant.
- This high variability renders the AgNOR quantitation method unsuitable for diagnostic or prognostic applications in breast lesions.