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Dimethyl sulfoxide induces programmed cell death and reversible G1 arrest in the cell cycle of human lymphoid pre-T
O Trubiani1, M Ciancarelli, M Rapino
1Istituto di Morfologia Umana Normale, Facoltà di Medicina, Università di Chieti, Italy.
Abstract:
In human B- and T-differentiated lymphoid cell lines DMSO was found to arrest the proliferation at the G1 stage of the cell cycle, without any detectable differentiation and DMSO itself was found to prevent apoptosis. Programmed cell death, or apoptosis, is now thought to be an important regulatory process in normal hemopoiesis and in the lymphoid system this program is started in the immune process such as autoreactive T-cell elimination in the thymus, and antigen-driven B-cell selection in the terminal centre. For this purpose, we have analysed the effect of DMSO using undifferentiated pre-B (KM-3) and pre-T (RPMI-8402) human lymphoid cells. Results obtained by multiparametric analyses show that DMSO affect only the pre-T cell line inducing a reversible G1 arrest of the cell cycle with a significant presence of apoptotic cells and modification of terminal transferase (TdT) expression. Pre-B cell line is resistant to DMSO treatment. These data provide evidence of a new model for the study of the selective cell type depending effect of DMSO in the immune system.
Insights
Dimethyl sulfoxide (DMSO) selectively impacts pre-T cells, causing cell cycle arrest and apoptosis, while leaving pre-B cells unaffected. This reveals a new model for studying DMSO
Area of Science:
- Immunology
- Cell Biology
- Pharmacology
Background:
- Programmed cell death (apoptosis) is crucial for immune system regulation, including T-cell elimination and B-cell selection.
- Dimethyl sulfoxide (DMSO) is known to affect cell proliferation and prevent apoptosis in some cell types.
Purpose of the Study:
- To investigate the differential effects of DMSO on human pre-B and pre-T lymphoid cell lines.
- To understand DMSO's role in regulating cell cycle progression and apoptosis in immune cells.
Main Methods:
- Utilized undifferentiated human pre-B (KM-3) and pre-T (RPMI-8402) lymphoid cell lines.
- Performed multiparametric analyses to assess cell cycle, differentiation, apoptosis, and terminal transferase (TdT) expression.
- Evaluated the reversibility of DMSO-induced effects.
Main Results:
- DMSO induced a reversible G1 cell cycle arrest specifically in the pre-T cell line.
- A significant increase in apoptotic cells was observed in the DMSO-treated pre-T cells.
- Terminal transferase (TdT) expression was modified in the pre-T cells following DMSO treatment.
- The pre-B cell line demonstrated resistance to DMSO's effects on proliferation and apoptosis.
Conclusions:
- DMSO exhibits selective effects on distinct immune cell precursors, impacting pre-T cells but not pre-B cells.
- These findings establish a novel model for studying cell-type-specific responses to DMSO within the immune system.
- The differential response highlights DMSO's potential as a tool to modulate specific immune cell populations.