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[The E test and quinolone-resistant Campylobacter jejuni]
R Cogollos1, J I Alós, J L Gómez-Garcés
1Servicio de Microbiología, Hospital de Móstoles, Instituto Nacional de Salud, Móstoles, Madrid.
Enfermedades Infecciosas Y Microbiologia Clinica
|December 1, 1995
Summary
The E-Test offers a viable alternative for monitoring antibiotic resistance in Campylobacter jejuni. While generally effective, its accuracy varies across different antibiotics compared to agar dilution methods.
Area of Science:
- Clinical Microbiology
- Antimicrobial Resistance
- Bacterial Pathogenesis
Background:
- Campylobacter jejuni infections are increasing, with growing antibiotic resistance.
- Antimicrobial sensitivity testing is crucial for guiding treatment decisions.
- The E-Test is an alternative method for determining antibiotic susceptibility.
Purpose of the Study:
- To compare the E-Test with agar dilution for antimicrobial susceptibility testing.
- To evaluate the E-Test's performance in ciprofloxacin-resistant Campylobacter jejuni strains.
- To assess the correlation between E-Test and agar dilution for 10 antimicrobial agents.
Main Methods:
- Studied 57 ciprofloxacin-resistant Campylobacter jejuni strains.
- Utilized the E-Test for antimicrobial sensitivity determination.
- Compared E-Test results with parallel agar dilution technique findings.
Main Results:
- High agreement between E-Test and agar dilution for ciprofloxacin and erythromycin (approx. 90%).
- Lower agreement for ampicillin, amoxicillin/clavulanic acid, and gentamicin (less than 30%).
- Discrepancies observed for clindamycin (1 strain) and tetracycline (8 strains).
Conclusions:
- The E-Test is a potentially valid method for tracking resistant Campylobacter jejuni.
- Correlation with agar dilution was discrete, indicating limitations.
- The cost of the E-Test may restrict its widespread adoption.