Related Experiment Video
Updated: Sep 3, 2026

Single Read and Paired End mRNA-Seq Illumina Libraries from 10 Nanograms Total RNA
Published on: October 27, 2011
Preparation of a differentially expressed, full-length cDNA expression library by RecA-mediated triple-strand
T B Hakvoort1, J A Spijkers, J L Vermeulen
1Department of Anatomy and Embryology, University of Amsterdam, The Netherlands.
Abstract:
We have developed a fast and general method to obtain an enriched, full-length cDNA expression library with subtractively enriched cDNA fragments. The procedure relies on RecA-mediated triple-helix formation of single-stranded cDNA fragments with a double-stranded cDNA plasmid library. The complexes were then captured from the solutions using the digoxigenin-antidigoxigenin paramagnetic beads followed by recovery of the enriched double-stranded cDNA expression library. We have observed a linear relation between the capture of full-length cDNAs in the library and the fold enrichment in the subtracted cDNA population.
More Related Videos
Related Concept Videos
Complementary DNA
RACE - Rapid Amplification of cDNA Ends
Since the...
RNA-seq
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...

