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A rapid polarographic immunoassay based on the anodic current of metal labeling
1Institute of Electroanalytical Chemistry, Northwest University, Xian, 710069, People's Republic of China.
Abstract:
A heterogeneous voltammetric immunoassay of human serum albumin (HSA) based on the anodic current of metal labeling was studied. HSA was covalently coupled to diethylenetriaminepentaacetic anhydride and labeled with In3+. A heterogeneous competitive immunochemical reaction was carried out in the wells of a microtiter plate. The In3+ label was released in 1 M HCl solution and determined by simplified anodic stripping neopolarography. HSA could be detected in the concentration range of 4.0-50 microg ml-1. The method was used to determine HSA in serum specimens, and the results were in good agreement with those obtained with dye binding spectrophotometry.