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Acylation of endogenous acyl acceptors by mouse sciatic nerve microsomes
H Juguelin1, J J Bessoule, F Boiron
1Laboratoire de Biogenése Membranaire, Université de Bordeaux 2-URA-CNRS 1811, France.
Neurochemistry International
|March 1, 1996
Summary
Mouse sciatic nerve microsomes can label phospholipids with fatty acids from acyl-CoAs without added acceptors. This phospholipid acylation involves an acyl exchange mechanism, primarily at position 2.
Area of Science:
- Biochemistry
- Molecular Biology
- Neuroscience
Background:
- Phospholipid metabolism is crucial for cellular function.
- Acyl-CoA is a key substrate in lipid synthesis.
- Microsomal fractions contain enzymes involved in lipid modification.
Purpose of the Study:
- To investigate phospholipid labeling in mouse sciatic nerve microsomes.
- To determine the role of acyl-CoAs in this process.
- To elucidate the mechanism of acylation.
Main Methods:
- Incubation of mouse sciatic nerve microsomes with radioactive oleoyl-CoA.
- Measurement of phospholipid and lysophosphatidylcholine labeling.
- Analysis of acyl group incorporation into specific positions of phospholipids.
Main Results:
- Phosphatidylcholine labeling occurred without added acyl acceptors.
- Maximal acylation was observed at low oleoyl-CoA concentrations.
- Acyl groups were primarily inserted at position 2 of glycerophosphorylcholine, forming lysophosphatidylcholine.
Conclusions:
- Mouse sciatic nerve microsomes exhibit intrinsic phospholipid acylation activity.
- The process appears to involve an acyl exchange mechanism between acyl-CoA and phosphatidylcholine.
- Acyl group insertion preferentially occurs at the sn-2 position.