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Antibody characteristics for a continuous response fiber optic immunosensor for theophylline
C M Hanbury1, W G Miller, R B Harris
1Department of Pathology and Biochemistry, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298-0597, USA.
Biosensors & Bioelectronics
|January 1, 1996
Summary
A new fiber optic immunosensor continuously measures theophylline levels. It uses fluorescence quenching and antibody binding for accurate, real-time detection of theophylline concentrations.
Area of Science:
- Biomedical Engineering
- Analytical Chemistry
- Immunosensor Technology
Background:
- Continuous monitoring of theophylline is crucial for therapeutic drug management.
- Existing methods may lack real-time capabilities or require complex sample handling.
Purpose of the Study:
- To develop a self-contained fiber optic immunosensor for continuous theophylline measurement.
- To optimize sensor parameters for enhanced dynamic range and reduced response time.
Main Methods:
- Utilized non-radiative energy transfer (NRET) between a B-phycoerythrin labeled theophylline donor and a Texas Red labeled antibody acceptor.
- Investigated monoclonal antibody selection criteria and optimized labeling conditions.
- Analyzed fluorescence quenching and antibody binding equilibrium shifts.
Main Results:
- Developed a sensor based on fluorescence quenching via NRET.
- Identified optimal antibody clones exhibiting significant quenching and reversible binding.
- Demonstrated a proportional increase in fluorescence with increasing free theophylline concentration.
Conclusions:
- The developed fiber optic immunosensor provides a viable method for continuous theophylline monitoring.
- Sensor performance is influenced by antibody kinetics and analyte diffusion rates.
- This technology offers potential for improved therapeutic drug monitoring.