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Cleavage specificity of coxsackievirus 3C proteinase for peptide substrate
K Miyashita1, J Okunishi, R Utsumi
1Central Research Laboratories, Maruishi Pharmaceutical Co., Ltd., Osaka, Japan.
Bioscience, Biotechnology, and Biochemistry
|April 1, 1996
Abstract:
The substrate requirements of coxsackievirus 3C proteinase (3Cpro) were investigated on the C-terminal side of the scissile bond using C-terminal truncated peptides of the substrate peptide Ac-EALFQGPPV. Not only the Gln-Gly bond of Ac-EALFQG-NH2 but also the C-terminal amide group of Ac-EALFQ-NH2 was hydrolyzed by 3Cpro, suggesting that the essential residues for cleavage by coxsackievirus 3Cpro would exist within the N-terminal 5 residues.