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Properties of nicked and circular dumbbell RNA-DNA chimeric oligonucleotides
H Yamakawa1, K Hosono, T Ishibashi
1Department of Industrial Chemistry, Chiba Institute of Technology, Japan.
Nucleic Acids Symposium Series
|January 1, 1995
Abstract:
We have designed a new type of antisense oligodeoxyribonucleotide. These oligonucleotides form two hairpin loop structures with RNA-DNA base pairs (sense (RNA) and antisense (DNA)) in the double helical stem. The nicked and circular dumbbell RNA/DNA chimeric oligonucleotides are molecules, with or without free ends, that are more resistant to exonuclease attack. Of particular interest, antisense DNA is liberated by RNase H treatment of the dumbbell RNA/DNA chimeric oligonucleotides.