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Dextran flux in M-K medium-stored human corneas
Investigative Ophthalmology & Visual Science
|August 1, 1977
Summary
Dextran molecules (over 40,000 molecular weight) can penetrate the corneal endothelium during storage in M-K medium. Penetration is influenced by storage duration and endothelial health.
Area of Science:
- Ophthalmology
- Corneal Science
- Biomaterials
Background:
- Corneal storage medium (M-K medium) is crucial for preserving donor corneas.
- Understanding the permeability of corneal endothelium to storage solution components is vital for optimizing preservation protocols.
Purpose of the Study:
- To investigate the permeability of the corneal endothelium to dextran molecules during storage.
- To determine factors influencing dextran penetration into the corneal endothelium.
Main Methods:
- Corneas were stored in M-K medium containing dextran (minimal molecular weight 40,000).
- Corneal endothelium permeability was assessed post-storage.
Main Results:
- Dextran with a minimal molecular weight of 40,000 was found to permeate the corneal endothelium.
- The extent of dextran penetration correlated with the duration of corneal storage.
- Endothelial condition significantly affected the degree of dextran penetration.
Conclusions:
- Corneal endothelium is permeable to dextran (≥40,000 MW) during M-K medium storage.
- Storage time and endothelial integrity are key determinants of dextran penetration.
- Findings have implications for optimizing corneal storage solutions and assessing endothelial viability.