In vitro detection of hydrogen peroxide in mesothelial cells

Hydrogen peroxide can be indirectly detected in mesothelial cells by measuring the rate of inhibition of the intracellular catalase activity by 3-amino-1, 2,3-triazole (AT). AT binds and inhibits catalase only in the presence of hydrogen peroxide, and the effect is proportional to the amount of hydrogen peroxide. The effect of AT on catalase activity is prevented in the presence of ethanol. In mesothelial cells in vitro exposed to dialysis fluids (Dianeal 1.36%, 2.27%, and 3.86%) no significantly increased generation of hydrogen peroxide was detected. However, interleukin-1 (IL-1) (10 ng/mL) enhances, within two hours (+40%, p < 0.05), intracellular production of hydrogen peroxide. The presented method of detection of intracellular hydrogen peroxide may be helpful in studies of the pathomechanisms causing mesothelial damage in conditions of peritoneal dialysis.