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The cellular patterns of BDNF and trkB expression suggest multiple roles for BDNF during Xenopus visual system
S Cohen-Cory1, E Escandón, S E Fraser
1Division of Biology, Beckman Institute, California Institute of Technology, Pasadena 91125, USA.
Developmental Biology
|October 10, 1996
Summary
Brain-Derived Neurotrophic Factor (BDNF) plays crucial roles in developing Xenopus visual systems. This study reveals BDNF
Area of Science:
- Neuroscience
- Developmental Biology
- Ophthalmology
Background:
- Brain-Derived Neurotrophic Factor (BDNF) and its receptor TrkB are implicated in visual system development.
- Previous studies suggest BDNF influences retinal ganglion cell survival and differentiation.
Purpose of the Study:
- To investigate the cellular expression sites of BDNF in the developing retina and optic tectum.
- To determine the roles of endogenous BDNF in retinal development and visual system formation.
Main Methods:
- In situ hybridization to detect BDNF and trkB gene expression.
- BDNF cross-linking studies to identify binding sites.
- Experiments using neutralizing antibodies to BDNF in retinal cell cultures.
Main Results:
- BDNF is expressed in the developing optic tectum and retina, including retinal ganglion cells, ciliary margin, and inner nuclear layer.
- BDNF expression correlates with trkB expression in retinal ganglion and amacrine cells.
- Neutralizing antibodies to BDNF reduce retinal ganglion cell survival and differentiation, while exogenous BDNF enhances it.
Conclusions:
- BDNF acts as a target-derived neurotrophin in the optic tectum.
- Endogenous BDNF in the retina supports retinal ganglion cell survival and differentiation.
- BDNF plays autocrine and/or paracrine roles in visual system development, in addition to its target-derived function.