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Updated: Oct 21, 2026

Preparation and Observation of Thick Biological Samples by Scanning Transmission Electron Tomography
Published on: March 12, 2017
Optical and digital image processing in high-resolution electron microscopy
Abstract:
A comparison is made of the deblurring by digital and optical processing of a single conventional bright field electron image of ferritin particles distributed on a carbon supporting film. The deblurring functions include the effects of the partial temporal and partial spatial coherence of the electron beam, the phase contrast transfer function of the objective lens and Weiner signal to noise optimisation. Practical aspects of the similar results achieved by the two methods are discussed. The relative advantages and disadvantages of the batch and interactive computer modes for picture processing are considered in an Appendix.
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