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Variability of platelet degranulation by different contrast media
E F Grabowski1, I K Jang, H Gold
1Massachusetts General Hospital-Harvard Medical School, Boston 02114, USA.
Academic Radiology
|September 1, 1996
Summary
Contrast media can affect blood platelets. This study found that platelet degranulation by contrast media is not dependent on whether the agent is ionic or nonionic, challenging previous suggestions.
Area of Science:
- Biomedical science
- Hematology
- Radiology
Background:
- Previous studies suggested nonionic contrast media, but not ionic, cause platelet degranulation.
- Flow cytometry has been used to assess contrast media effects on platelets.
Purpose of the Study:
- To investigate whether contrast media ionic or nonionic nature influences platelet degranulation.
- To assess platelet function using whole-blood platelet flow cytometry and flowing blood platelet aggregometry.
Main Methods:
- Blood samples from six healthy donors were mixed with ionic (diatrizoate, ioxaglate) and nonionic (iohexol, iodixanol) contrast media.
- Platelet function was assessed using flow cytometry and aggregometry under different anticoagulant conditions.
- Samples were fixed for analysis within 30 minutes.
Main Results:
- Platelet degranulation was observed with iohexol (nonionic) and diatrizoate (ionic).
- No degranulation was observed with iodixanol (nonionic) or ioxaglate (ionic).
Conclusions:
- Platelet degranulation by contrast media is independent of the agent's ionic or nonionic properties.
- The findings suggest other factors may be responsible for contrast media-induced platelet activation.