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Hybridization and detection of digoxigenin probes on RNA blots
1University of Michigan-Dearborn 48128, USA. egodsall@cw-fl.umd.umich.edu
Molecular Biotechnology
|August 1, 1996
Abstract:
This article describes nonradioactive probing of a Northern blot. The method employs digoxigenin-labeled probes. Antidigoxigenin antibody/alkaline phosphate conjugate, and a chemiluminescent substrate are subsequently used in the detection system.