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Simple procedure for estimating the efficiency of PCR
A S Bourinbaiar1, K Ajuang-Simbiri
1Metatron Inc., New York, NY 10003, USA.
Molecular Biotechnology
|August 1, 1996
Abstract:
The extension efficiency of PCR-driven DNA amplification was examined by step-controlled limiting dilution method using as internal controls 8E5 lymphocytes carrying a single copy of HIV genome. The results reveal that under standard conditions recommended by the PCR kit manufacturer the exponential growth of DNA template is approximately one third of the theoretical doubling rate.
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