Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Validation procedures for the Hamilton Thorne Integrated Visual Optical System sperm and cell analyzer

D H Douglas-Hamilton1

  • 1Hamilton Thorne Research, Beverly, MA 01915, USA.

Quality Assurance (San Diego, Calif.)
|December 1, 1995
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

CO(2) (10.6-microm) atmospheric propagation enhancement due to off-line center tuning.

Applied optics·2010
Same author

Transmission at lambda = 10.6-mum wavelength through the upper atmosphere.

Applied optics·2010
Same author

Thermal effects in laser-assisted pre-embryo zona drilling.

Journal of biomedical optics·2001
Same author

A field study of the fertility of transported equine semen.

Theriogenology·1984
Same author

Moral periodic table.

Science (New York, N.Y.)·1976
Same journal

Peer Review as a QA Tool in Environmental Applications.

Quality assurance (San Diego, Calif.)·2006
Same journal

The use of double anonymity in peer review: a decision whose time has come?

Quality assurance (San Diego, Calif.)·2006
Same journal

Major issues in quality assessment and improvement of clinical hyperbaric services: an international perspective.

Quality assurance (San Diego, Calif.)·2006
Same journal

Evaluation of customer satisfaction level of different projects.

Quality assurance (San Diego, Calif.)·2006
Same journal

Establishing tolerance levels for customer complaints.

Quality assurance (San Diego, Calif.)·2006
Same journal

The historical impact of ISO 9000 on Lebanese firms.

Quality assurance (San Diego, Calif.)·2005
See all related articles

The Integrated Visual Optical System (IVOS) analyzer accurately measures sperm parameters in lab animals for reproductive toxicology. Its digital system ensures reliable, validated results for on-site analysis, meeting Good Laboratory Practices.

Area of Science:

  • Reproductive Toxicology
  • Sperm Analysis
  • Laboratory Animal Science

Background:

  • Sperm analysis is crucial in reproductive toxicology studies.
  • Accurate measurement of sperm motility and concentration is essential for reliable results.
  • Existing methods require validation for on-site application and Good Laboratory Practices (GLP) compliance.

Purpose of the Study:

  • To evaluate the Hamilton Thorne Integrated Visual Optical System (IVOS) analyzer for sperm analysis in laboratory animals.
  • To assess the accuracy and reliability of the IVOS system for measuring sperm motility and concentration.
  • To determine the system's suitability for on-site validation according to GLP standards.

Main Methods:

  • The IVOS system, combining optical and computer analysis, was used to assess sperm motility, progressive motility, velocity, and concentration.

Related Experiment Videos

  • Digital encoding allowed for image storage and absolute replay fidelity for reexamination.
  • Validation involved a NIST-certified scale for length standards and manual confirmation of parameters using Cartesian coordinates.
  • Sperm concentration was determined using a DNA stain to differentiate sperm from somatic cells.

    • Main Results:

    • The IVOS system demonstrated high agreement (within 0.2%) between manual and computed sperm motion parameters.
    • Sperm concentration measurements by IVOS in rat testis and epididymis homogenates were within 5% of manual hemacytometer counts.
    • The system facilitates on-site validation through digital image storage and replay capabilities.

    Conclusions:

    • The Hamilton Thorne IVOS analyzer is a validated tool for accurate sperm analysis in laboratory animals.
    • The system's features support on-site validation and compliance with Good Laboratory Practices.
    • IVOS provides reliable measurements of sperm motility and concentration, crucial for reproductive toxicology research.