Review: in vitro spermicidal tests

This review analyzes in vitro spermicidal tests that have been used to evaluate the spermicidal activity of contraceptive products. Special requirements and processes in numerous technologies were also reviewed. Nonoxynol-9 (N-9) was used as an example of a vaginally delivered spermicidal agent, and its spermicidal potency was compared by various spermicidal tests, such as Sander-Cramer, computer-assisted semen analysis, hypoosmotic swelling test, cervical mucus penetration test, and flow cytometry, and advantages and disadvantages of each test were specified. This provides an insight into the different aspects of sperm functionality on which each spermicidal agent exerts its activity. A rationale of the best combination of in vitro spermicidal tests, with particular emphasis on a simple and efficient strategy that targets the complete fertility control, was explained.