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Updated: Aug 11, 2026
Establishment of a Clinically Relevant Ex Vivo Mock Cataract Surgery Model for Investigating Epithelial Wound Repair in a Native Microenvironment
Published on: June 5, 2015
An in vivo model for evaluating wound repair and regeneration microenvironments
1Department of Surgery, University of Minnesota, Minneapolis 55455, USA. sicar001@maroon.tc.umn.edu
Background:
Factors differentially regulating regeneration and repair as contrasting resolutions to injury are unknown. We adapted a common wound healing model to further characterize mammalian wound repair and regeneration microenvironments.
Methods:
Polyvinyl alcohol sponges, sponges containing minced muscle, or blocks of minced muscle were implanted onto the backs of Fischer rats. Vascularization was assessed by infusion with india ink and progress of regeneration was evaluated histologically.
Results:
Regeneration occurring within sponges was histologically similar to that of free muscle blocks; but, was initiated more slowly. Vascularization of minced muscle implanted in sponges was delayed slightly compared to implanted free muscle blocks.
Conclusions:
Regeneration of minced muscle in sponges parallels normal regeneration of free minced muscle. Incorporation into a wound repair model provides access to interstitial fluids conditioned by regenerating muscle and will lead to more detailed comparisons of the content and properties of repair and regeneration microenvironments.
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