High-frequency endonuclease (REA) typing: results from the WHO collaborative study group on subtyping of Listeria

¹Department of Clinical Microbiology, Statens Seruminstitut, Copenhagen S, Denmark.

International Journal of Food Microbiology

|October 1, 1996

Abstract:

Eighty isolates of Listeria monocytogenes were typed by high-frequency restriction endonuclease analysis. Two laboratories participated in the study with two restriction enzymes each. The enzymes used were EcoRI, Hae1II, HhaI. and its isoschizomer Cfo1. EcoRI was less discriminatory than the other enzymes. The profiles generated by Hha1 and CfoI were not fully stable for some closely related isolates. The size and the number of restriction bands generated by Hha1 in one laboratory and its isoschizomer Cfo1 in another laboratory were directly comparable. This indicates that REA-typing may be used as a definitive typing method for Listeria monocytogenes if the typing procedure is standardized. The stability of the REA-types needs further elucidation in order to establish firm differentiation criteria for comparison of isolates.

Related Concept Videos

Articles linked to this work by shared authors, journal, and citation graph.

Azole resistance by loss of function of the sterol Δ⁵,⁶-desaturase gene (ERG3) in Candida albicans does not necessarily decrease virulence.

Antimicrobial agents and chemotherapy·2012

Multiple resistance mechanisms to azole antifungals in yeast clinical isolates.

Drug resistance updates : reviews and commentaries in antimicrobial and anticancer chemotherapy·2006

Role of ATP-binding-cassette transporter genes in high-frequency acquisition of resistance to azole antifungals in Candida glabrata.

Antimicrobial agents and chemotherapy·2001

The ATP binding cassette transporter gene CgCDR1 from Candida glabrata is involved in the resistance of clinical isolates to azole antifungal agents.

Antimicrobial agents and chemotherapy·1999

Amino acid substitutions in the cytochrome P-450 lanosterol 14alpha-demethylase (CYP51A1) from azole-resistant Candida albicans clinical isolates contribute to resistance to azole antifungal agents.

Antimicrobial agents and chemotherapy·1998

Susceptibilities of Candida albicans multidrug transporter mutants to various antifungal agents and other metabolic inhibitors.

Antimicrobial agents and chemotherapy·1996

See all related articles

Related Experiment Videos

High-frequency endonuclease (REA) typing: results from the WHO collaborative study group on subtyping of Listeria

Related Concept Videos

Azole resistance by loss of function of the sterol Δ⁵,⁶-desaturase gene (ERG3) in Candida albicans does not necessarily decrease virulence.

Multiple resistance mechanisms to azole antifungals in yeast clinical isolates.

Role of ATP-binding-cassette transporter genes in high-frequency acquisition of resistance to azole antifungals in Candida glabrata.

The ATP binding cassette transporter gene CgCDR1 from Candida glabrata is involved in the resistance of clinical isolates to azole antifungal agents.

Amino acid substitutions in the cytochrome P-450 lanosterol 14alpha-demethylase (CYP51A1) from azole-resistant Candida albicans clinical isolates contribute to resistance to azole antifungal agents.

Susceptibilities of Candida albicans multidrug transporter mutants to various antifungal agents and other metabolic inhibitors.

First report of PLSA resistance gene sal(B) in multidrug-resistant foodborne Mammaliicoccus lentus: Molecular characterization and food safety implications.

Grape ripeness shapes microbial community dynamics and fermentation outcomes during modified pied de cuve wine fermentations.

The impact of beer-spoiling yeast on flavour development in non-alcoholic beers.

Synthetic community producing bacterial inhibitory proteins to control postharvest black spot disease of winter jujube.

Quantitative and genomic epidemiology of Staphylococcus aureus in retail foods: insights into foodborne risk and clinically relevant lineages.

Combating colistin- and cefotaxime-resistant Shiga toxin-producing Escherichia coli in dairy-based products: Occurrence, characterization, and ε-poly-L-lysine growth inactivation.

A Classification Algorithm for Diabetes Subtypes in a Swedish Cohort: Comparison With National Diabetes Register Diagnoses and Genetic Validation.

Spontaneous Achromatopsia-Like Phenotype in Albino Swiss CD-1 Mice: Retinocortical Dysfunction, Neurovascular Remodeling, and Glial Activation.

Clinical Implications of the Novel Genetic Polymorphism Associated With Retinal Vein Occlusion: The East Asian GWAS Survey in a Single-Center Taiwanese Cohort.

Acute Hypoxia Induces Cardiomyocyte Autophagy via SIRT5/mTOR/ULK1 Pathway.

Implementation of next-generation sequencing to guide care and public health action in settings with high burden of rifampicin-resistant tuberculosis: 32 stakeholder consensus statements generated using Delphi principles.

Unbiased Long-Read Whole-Genome Sequencing Enables High-Resolution Mapping of Transgene Concatenation and Off-target Genomic Disruption in a Mouse Model.

Related Experiment Videos

High-frequency endonuclease (REA) typing: results from the WHO collaborative study group on subtyping of Listeria...

Abstract:

Related Concept Videos

Related Articles

High-frequency endonuclease (REA) typing: results from the WHO collaborative study group on subtyping of Listeria