[Use and preparation of thrombocyte concentrates]

Blood component transfusion therapy is an achievement of up-to-date transfusiology and is possible due to plastic equipment, centrifuge with air conditioning and modern technology which enables splitting blood into its components. Platelet concentration is a preparation of platelets gained from one unit of the whole blood and is used in therapy of patients with thrombocytopenias and thrombocytopathies. It can be preserved, depending on plastic bags, from 1 to 5 days with rotation on 22 degrees Celsius. This paper presents two methods of platelet concentration preparation (out of thrombocytes rich plasma and buffy coat) contribution of thrombocytes in concentrations and contamination of concentration with leucocytes, as well as changes during 72 hours of concentration preservation. One hundred units of thrombocytes had an average volume 51.42 ml. Platelet concentration gained out of thrombocyte rich plasma was 0.98 x 1011/l, and 0.80 x 109/l leucocytes. The number of thrombocytes in concentrations from buffy coat was 0.82 x 101/l, and 0.10 x 109/l leucocytes. Plasma pH after preparation was 7.21, and three days of conservation later it was 6.68. During these 72 hours of preserving the concentration there has not been a significant decrease in regard to the number of thrombocytes.