Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Epithelial cell migration in the normal rat lens

J Pe'er1, M Muckarem, G Zajicek

  • 1Department of Ophthalmology, Hadassah University Hospital, Jerusalem, Israel.

Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft
|October 1, 1996
PubMed
Summary

Researchers estimated rat lens epithelium cell displacement, finding progenitor cells move at 21.8 microns/day. This movement pattern is similar to renewing tissues like epidermis.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Stable knockdown of CREB, HIF-1 and HIF-2 by replication-competent retroviruses abrogates the responses to hypoxia in hepatocellular carcinoma.

Cancer gene therapy·2016
Same author

Computerized extraction of the time dimension in histopathological sections.

Applied optics·2010
Same author

Ruthenium-106 plaque brachytherapy for thick posterior uveal melanomas.

The British journal of ophthalmology·2009
Same author

Long-term survival of uveal melanoma patients after surgery for liver metastases.

The British journal of ophthalmology·2009
Same author

Lesions of the caruncle: a description of 42 cases and a review of the literature.

Eye (London, England)·2008
Same author

Intravitreal methotrexate for treating vitreoretinal lymphoma: 10 years of experience.

The British journal of ophthalmology·2008

Area of Science:

  • Ophthalmology
  • Cell Biology
  • Developmental Biology

Background:

  • The lens epithelium is crucial for maintaining lens clarity and function.
  • Understanding cell dynamics within the lens is key to studying ocular development and disease.
  • Previous research has not precisely quantified the rate of cell displacement in the rat lens epithelium.

Purpose of the Study:

  • To estimate the rate of cell displacement in the rat lens epithelium.
  • To characterize the kinetic behavior of lens epithelial cells.
  • To compare lens epithelium cell dynamics to other renewing tissues.

Main Methods:

  • Adult male rats were injected with [3H] thymidine for cell proliferation tracing.
  • Eyes were collected at various time points (1 hour to 21 days) post-injection.

Related Experiment Videos

  • Autoradiography and microscopy were used to quantify cell position and grain content, with statistical analysis including linear regression.

    • Main Results:

    • Lens epithelial cells were categorized into progenitor (P-cells) and non-proliferating (Q-cells) compartments.
    • Cells moved from the P-compartment (fields 9-20) to the Q-compartment (fields 0-8) at an average velocity of 21.8 microns/day.
    • No significant cell movement was observed between field 20 and the lens center (field 34).

    Conclusions:

    • The rat lens epithelium exhibits a distinct pattern of cell displacement, with progenitor cells migrating at a quantifiable rate.
    • These findings suggest the lens epithelium functions as a kinetically renewing tissue, analogous to the epidermis and gastrointestinal mucosa.
    • This study provides a quantitative basis for understanding lens epithelial cell dynamics and renewal processes.