Estimation of composition of weight loss in man: a comparison of methods

Five different procedures were used to estimate composition of weight loss by six obese individuals maintained on low-calorie regimens: 1) energy-nitrogen (E-N) balance; 2) total body water-nitrogen balance (TBW-N); 3) total body water (TBW); 4) total body potassium (TBK); and 5) an anthropometric method. Quantity of weight lost by obese subjects during comparison periods lasting 25-40 days ranged from 7.9 to 17.1 kg. Expressed as percent of aggregate weight loss, collective fat loss was 47.6 by E-N balance; 46.3 by TBW-N balance; 51.7 by TBK; 50.9 by anthrometry; and 33 by TBW. The E-N balance method yielded a tight cluster of individual means, whereas the other procedures gave a wide scatter of means. For short-term studies, the E-N balance procedure, although tedious and difficult, should yield results less variable than those generated by any of the other methods examined.