Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Pore-like structures in biological membranes

L Orci, A Perrelet, F Malaisse-Lagae

    Journal of Cell Science
    |June 1, 1977
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    THE STIMULUS-SECRETION COUPLING OF GLUCOSE-INDUCED INSULIN RELEASE : IV. The Effect of Vincristine and Deuterium Oxide on the Microtubular System of the Pancreatic Beta Cell.

    The Journal of cell biology·2009
    Same author

    The oxygen concentrator: an appropriate technology for treating hypoxaemic children in developing countries.

    The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease·2004
    Same author

    Pancreatic fate of D-[3H] mannoheptulose.

    Cell biochemistry and function·2001
    Same author

    Labeling of pancreatic glycogen by D-[U-14c]glucose in hyperglycemic rats.

    Endocrine·2001
    Same author

    Pancreatic fate of a (125)I-labelled mouse monoclonal antibody directed against pancreatic B-cell surface ganglioside(s) in control and diabetic rats.

    Cell biochemistry and function·2001
    Same author

    Pancreatic uptake of [2-(14)C]alloxan.

    International journal of molecular medicine·2001
    Same journal

    Potential role of myeloid bodies in protection against photo-oxidative damage of the retinal pigment epithelium.

    Journal of cell science·2026
    Same journal

    From the FAZ to the TAC: Widespread cytoskeletal engagement of T. brucei KMP11.

    Journal of cell science·2026
    Same journal

    Branched actin segregates endocytic cargo to control sorting and fission.

    Journal of cell science·2026
    Same journal

    FAM122A inhibition of PP2A-B55 through a bipartite binding mechanism.

    Journal of cell science·2026
    Same journal

    Disentangling the response to lysosomal damage.

    Journal of cell science·2026
    Same journal

    The force, form and function of the nucleus.

    Journal of cell science·2026
    See all related articles

    Freeze-fracture electron microscopy reveals that intramembrane particles in biological membranes may contain hydrophilic channels. These channels in random particles could explain membrane permeability and water-filled pores.

    Area of Science:

    • Cell Biology
    • Membrane Biophysics
    • Electron Microscopy

    Background:

    • Biological membranes are composed of a lipid bilayer with embedded proteins, visualized as intramembrane particles in freeze-fracture replicas.
    • Intramembrane particles represent membrane proteins, while smooth areas are the lipid domains.
    • Previous studies on gap junction particles suggested they form hydrophilic channels.

    Purpose of the Study:

    • To investigate the structure of intramembrane particles in various cell types.
    • To determine if non-junction intramembrane particles also contain structures indicative of channels.
    • To correlate observed structures with models of membrane permeability.

    Main Methods:

    • Freeze-fracture electron microscopy of diverse cell types.

    Related Experiment Videos

  • Analysis of intramembrane particle morphology, focusing on electron-dense spots.
  • Comparative analysis between gap junction particles and other intramembrane particles.

    • Main Results:

    • A significant number of intramembrane particles across various cell types exhibit an electron-dense spot.
    • This spot is interpreted as a pit filled with platinum during sample preparation.
    • Comparison suggests these spots, similar to gap junction particles, may represent hydrophilic channels.

    Conclusions:

    • Random intramembrane particles, like those in gap junctions, may contain hydrophilic channels.
    • These channels could be the morphological basis for water-filled pores in membrane permeability models.
    • This finding offers structural insight into membrane transport mechanisms.