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Cell-volume regulation: P-glycoprotein--a cautionary tale
1Cystic Fibrosis Research Laboratory, Stanford University, California 94305-2130, USA.
Current Biology : CB
|November 1, 1996
Summary
P-glycoprotein is not the volume-sensitive organic anion channel (VSOAC) but may play a role in cell volume regulation after swelling. Its physiological significance remains uncertain.
Area of Science:
- Cell biology
- Physiology
- Molecular biology
Background:
- The volume-sensitive organic anion channel (VSOAC) is a key regulator of cell volume.
- P-glycoprotein is a well-known efflux pump with diverse cellular functions.
Purpose of the Study:
- To investigate whether P-glycoprotein functions as VSOAC.
- To determine the role of P-glycoprotein in cell volume regulation.
Main Methods:
- Electrophysiological recordings in cells expressing P-glycoprotein.
- Cell volume measurements following osmotic challenges.
Main Results:
- P-glycoprotein does not exhibit the characteristics of VSOAC.
- P-glycoprotein influences cell volume recovery after swelling.
Conclusions:
- P-glycoprotein is not VSOAC.
- The physiological role of P-glycoprotein in cell volume recovery requires further investigation.