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ATP-MgCL2 reduces intestinal permeability during mesenteric ischemia
P B Kreienberg1, R C Darling, D M Shah
1Department of Surgery, Section of Vascular Surgery, Neil Hellman Medical Research Building, Albany Medical College, 47 New Scotland Avenue, Albany, New York, 12208, USA.
The Journal of Surgical Research
|November 1, 1996
Summary
Adenosine triphosphate-magnesium chloride (ATP-MgCl2) prevents increased intestinal permeability during ischemia. This protective effect occurs without altering tissue ATP levels or increasing blood flow in the affected ileum.
Area of Science:
- Physiology
- Gastroenterology
- Pharmacology
Background:
- Organ ischemia can lead to increased intestinal permeability.
- Adenosine triphosphate-magnesium chloride (ATP-MgCl2) has shown protective effects in various ischemia models.
- The specific impact of ATP-MgCl2 on ileal permeability during ischemia requires further investigation.
Purpose of the Study:
- To investigate the efficacy of ATP-MgCl2 in reducing plasma to lumen permeability in ischemic rat ileum.
- To assess the effect of ATP-MgCl2 on intestinal blood flow and tissue adenosine triphosphate (ATP) levels during ischemia.
Main Methods:
- Ileal segments from rats were subjected to ischemia and perfused to measure 51Cr-EDTA clearance.
- Blood flow was quantified using labeled microspheres.
- Tissue ATP levels were determined enzymatically.
- Rats were pretreated with ATP-MgCl2 or a placebo.
Main Results:
- Ischemia significantly increased ileal permeability and reduced blood flow.
- ATP-MgCl2 pretreatment prevented the ischemia-induced increase in permeability.
- ATP-MgCl2 did not significantly alter tissue ATP levels or intestinal blood flow compared to untreated ischemic segments.
- Ischemia led to a significant reduction in tissue ATP levels.
Conclusions:
- ATP-MgCl2 effectively mitigates the increase in intestinal permeability caused by ischemia.
- The protective mechanism of ATP-MgCl2 in this context does not appear to involve direct increases in tissue ATP levels or blood flow.
- These findings suggest a potential therapeutic role for ATP-MgCl2 in managing intestinal ischemia.