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Structure and functional analysis of the multistress response gene DDR2 from Saccharomyces cerevisiae

N Kobayashi1, T K McClanahan, J R Simon

  • 1Laboratory of Structural Biology and Molecular Medicine, UCLA School of Medicine 90024, USA.

Biochemical and Biophysical Research Communications
|December 13, 1996
PubMed

Insights

The DDR2 gene in yeast responds to many stresses but its disruption does not affect heat shock, DNA damage, or mutagenesis. This suggests DDR2

Area of Science:

  • Molecular biology
  • Yeast genetics

Background:

  • The DDR2 gene in Saccharomyces cerevisiae is a known multistress response gene.
  • It is transcriptionally activated by diverse xenobiotic agents and various stresses.

Purpose of the Study:

  • To investigate the function of the DDR2 gene.
  • To characterize the phenotype of DDR2 gene disruption mutants.

Main Methods:

  • Construction of disruption alleles for the DDR2 gene.
  • Phenotypic analysis of ddr2 delta mutants.

Main Results:

  • DDR2 gene disruption mutants (ddr2 delta) showed no defects in heat shock recovery.
  • Thermotolerance was not affected in the ddr2 delta mutants.
  • DNA damage sensitivity and mutagenesis appeared normal in the absence of the DDR2 gene.

Conclusions:

  • The DDR2 gene's role in stress response may not be essential for basic thermotolerance or DNA repair/mutagenesis in yeast.
  • Further research is needed to elucidate the specific functions of DDR2 under various stress conditions.

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