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Susceptibility of gram-negative bacteria isolated from a paediatric hospital environment to antibiotics
E Zembrzuska-Sadkowska1, W Parnowska, B Werakso
1Drug Institute, Warsaw, Poland.
Abstract:
The majority of tested Gram-negative strains isolated from the hospital environment were resistant to 6-14 used antibiotics. The greatest resistance was shown by three strains: Enterobacter cloacae, Proteus mirabilis and Ps. maltophilia, which were resistant to all tested drugs. The most effective antibiotic was gentamicin, at least against half of bacteria belonging to Enterobacteriaceae family, to 90% of strains from Pseudomonas genus and to all other Gram-negative rods. Cephtazidim, cephotaxim, colistin and carbenicillin were effective only to 60-70% of Enterobacteriaceae family strains, whereas ampicillin and tetracycline to 70% of Pseudomonas genus. Other Gram-negative bacilli were more susceptible to antibiotics. Cephalothin was ineffective to all tested strains.
Insights
Most hospital-acquired Gram-negative bacteria show significant antibiotic resistance. Gentamicin proved most effective against these resistant strains, offering a potential treatment option.
Area of Science:
- Microbiology
- Infectious Diseases
- Pharmacology
Background:
- Hospital environments harbor numerous Gram-negative bacteria.
- Antibiotic resistance in these bacteria poses a significant clinical challenge.
Purpose of the Study:
- To assess the antibiotic resistance patterns of Gram-negative bacteria isolated from a hospital setting.
- To identify effective antibiotics against these resistant strains.
Main Methods:
- Isolation and identification of Gram-negative bacterial strains from hospital environments.
- Antibiotic susceptibility testing using a panel of 14 antibiotics.
- Evaluation of resistance prevalence and identification of the most effective antimicrobial agents.
Main Results:
- The majority of Gram-negative strains exhibited resistance to 6-14 antibiotics.
- Enterobacter cloacae, Proteus mirabilis, and Pseudomonas (Ps.) maltophilia showed resistance to all tested antibiotics.
- Gentamicin was the most effective antibiotic, showing activity against at least 50% of Enterobacteriaceae, 90% of Pseudomonas, and all other Gram-negative rods.
- Cephalothin was ineffective against all tested strains.
Conclusions:
- Significant multidrug resistance is prevalent among Gram-negative bacteria in hospital settings.
- Gentamicin demonstrates broad-spectrum efficacy against a wide range of resistant Gram-negative pathogens.
- Targeted antibiotic strategies are crucial for managing infections caused by resistant Gram-negative bacteria.