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Known HPV types have no association with keratoacanthomas
1MediCity Research Laboratory, Faculty of Medicine, University of Turku, Finland.
Archives of Dermatological Research
|March 1, 1996
Summary
This study found no evidence of common human papillomavirus (HPV) types in keratoacanthomas (KAs) using in situ hybridization. Limited PCR results suggest a potential link to HPV type 17, but further research is needed.
Area of Science:
- Dermatology
- Virology
- Oncology
Background:
- Human papillomavirus (HPV) is implicated in various skin conditions.
- The role of HPV in keratoacanthoma (KA) development remains controversial.
- Previous studies have yielded conflicting results regarding HPV and KAs.
Purpose of the Study:
- To investigate the presence of specific HPV types in keratoacanthoma (KA) biopsies.
- To clarify the potential role of HPV in the etiology of KAs.
- To analyze HPV DNA in paraffin-embedded KA specimens.
Main Methods:
- In situ hybridization (ISH) was performed on 80 KA biopsies using probes for 11 HPV types.
- Polymerase chain reaction (PCR) with consensus primers targeting HPV E1 and L1 regions was used on a subset of samples.
- Direct DNA sequencing was employed for PCR-positive products.
Main Results:
- All 80 specimens were negative for the targeted HPV types by ISH.
- PCR detected HPV L1 region DNA in 3 out of 20 cases, but not the E1 region.
- DNA sequencing revealed 76% homology with HPV type 17 in the positive PCR samples.
Conclusions:
- Commonly tested HPV types are unlikely to be involved in the etiology of keratoacanthomas.
- The findings do not support a significant role for known HPV types in KA development.
- Further investigation into less common HPV types or other etiological factors may be warranted.