Jove
Visualize
Contact Us

Related Experiment Videos

Sequences of two expressed sequence tags (EST) from rice encoding different cap-binding proteins

E Aliyeva1, A M Metz, K S Browning

  • 1Department of Chemistry and Biochemistry, University of Texas at Austin 78712, USA.

Gene
|November 21, 1996
PubMed
Summary

Researchers identified rice cap-binding proteins involved in translation initiation. These proteins are similar to those found in wheat, aiding in understanding plant translation mechanisms.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Control of protein translation by phosphorylation of the mRNA 5'-cap-binding complex.

Biochemical Society transactions·2007
Same author

Plant translation initiation factors: it is not easy to be green.

Biochemical Society transactions·2004
Same author

A plant viral "reinitiation" factor interacts with the host translational machinery.

Cell·2001
Same author

The RNA world in plants: post-transcriptional control III.

The Plant cell·2001
Same author

Two telomerase reverse transcriptases (TERTs) expressed in Candida albicans.

Biotechnology and applied biochemistry·2001
Same author

eIF4G functionally differs from eIFiso4G in promoting internal initiation, cap-independent translation, and translation of structured mRNAs.

The Journal of biological chemistry·2001
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Area of Science:

  • Molecular Biology
  • Plant Science
  • Biochemistry

Background:

  • Cap-binding proteins are crucial for translation initiation in eukaryotes.
  • Wheat possesses two forms of cap-binding proteins involved in this process.

Purpose of the Study:

  • To identify and characterize cap-binding proteins in rice, a higher plant species.
  • To compare rice cap-binding proteins with those from wheat and other organisms.

Main Methods:

  • Searched the expressed sequence tag (EST) database for rice sequences similar to wheat cap-binding proteins.
  • Obtained and completed cDNA sequences for identified rice ESTs.
  • Deduced amino acid sequences for comparative analysis.

Main Results:

Related Experiment Videos

  • Identified several rice ESTs with similarity to wheat cap-binding proteins.
  • Confirmed and characterized two rice cap-binding proteins.
  • Compared the deduced amino acid sequences of rice proteins with homologous proteins from wheat, yeast, fruit flies, frogs, and humans.

Conclusions:

  • Rice has cap-binding proteins homologous to those in wheat, suggesting conserved roles in translation initiation.
  • Comparative analysis provides insights into the evolution and function of cap-binding proteins across different species.