[Rosette formation in explants of human embryonic neocortex (an electron-microscopic study)]

Area of Science:

• Neuroscience
• Developmental Biology
• Cell Biology

Background:

• Rosette formation is a key process in early human brain development.
• Previous research suggested similarities between rosette formation and neurulation mechanisms.

Purpose of the Study:

• To investigate the cellular mechanisms underlying rosette formation in the human anterior cerebral vesicle.
• To confirm the proposed similarity between rosette formation and neurulation.
• To study the role of radial glia cells and neuroblasts in neocortex germ reorganization.

Main Methods:

• Analysis of 199 fragments of the human anterior cerebral vesicle wall.
• Observation of cell behavior and structural changes during rosette development.

Main Results:

• Multiple invaginations and closed cavities (rosettes) were observed in the cerebral vesicle wall fragments.
• Contraction of neuroepithelial cell apices, resembling a 'gathered tobacco pouch,' was identified as a key mechanism.
• The findings support the hypothesis that rosette formation shares mechanisms with neurulation.

Conclusions:

• Neuroepithelial cell apex contraction is crucial for rosette formation.
• Rosette formation in the anterior cerebral vesicle is mechanistically similar to neurulation.
• Radial glia cells and neuroblasts actively participate in neocortex germ reorganization.