Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Mixture analysis using a quadrupole mass filter/quadrupole ion trap mass spectrometer

K R Jonscher1, J R Yates

  • 1Department of Molecular Biotechnology, University of Washington, Seattle 98195-7730, USA.

Analytical Chemistry
|February 15, 1996
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Lorentz sphere visualised.

The Journal of chemical physics·2019
Same author

Lonomia obliqua bristle extract modulates Rac1 activation, membrane dynamics and cell adhesion properties.

Toxicon : official journal of the International Society on Toxinology·2019
Same author

Individual differences in impulsive action and dopamine transporter function in rat orbitofrontal cortex.

Neuroscience·2015
Same author

ProLuCID: An improved SEQUEST-like algorithm with enhanced sensitivity and specificity.

Journal of proteomics·2015
Same author

Global quantitative analysis of phosphorylation underlying phencyclidine signaling and sensorimotor gating in the prefrontal cortex.

Molecular psychiatry·2015
Same author

Effects of methylprednisolone and 4-chloro-3-hydroxyanthranilic acid in experimental spinal cord injury in the guinea pig appear to be mediated by different and potentially complementary mechanisms.

Spinal cord·2014
Same journal

The ACS at 150: The History of Analytical Chemistry Publications and a Century of Progress.

Analytical chemistry·2026
Same journal

Machine Learning-Enabled Image Analysis of Complex Chemical Mixtures: Synthetic Urine Droplets as a Test System.

Analytical chemistry·2026
Same journal

H<sub>2</sub>O<sub>2</sub>/Viscosity Tandem-Locked Fluorescent Probes Based on an In Situ Fluorophore Synthesis Strategy for Colitis Imaging and Diagnosis.

Analytical chemistry·2026
Same journal

TopoStitcher: A Geometric-Topological Structure-Guided Stitching Framework for Single-Molecule Localization Microscopy.

Analytical chemistry·2026
Same journal

Noninvasive SERS Immunosensing of Tyrosinase for Melanoma Monitoring via Microneedle Sampling Integrated with Satellite-Structured Bifunctional Nanozymes.

Analytical chemistry·2026
Same journal

Label-Free Electrochemical CRISPR Platform Gated by Allosteric Transcription Factors for Ultrasensitive Small-Molecule Detection.

Analytical chemistry·2026
See all related articles

A new hybrid tandem mass spectrometer improves peptide analysis by combining a quadrupole mass filter with an ion trap. This novel instrument enhances molecular weight, fragmentation, and high-resolution measurements, offering superior performance.

Area of Science:

  • Analytical Chemistry
  • Mass Spectrometry
  • Instrumentation

Background:

  • Quadrupole ion trap mass spectrometers (QITMS) are versatile analytical tools.
  • Interfacing mass filters with ion traps can enhance analytical capabilities.
  • Previous limitations in QITMS performance include space charge effects and resolution.

Purpose of the Study:

  • To construct and evaluate a hybrid tandem mass spectrometer by interfacing a quadrupole mass filter (Q) with a QITMS.
  • To assess the instrument's performance for analyzing complex mixtures.
  • To compare the hybrid system's capabilities against a standalone QITMS.

Main Methods:

  • Construction of a hybrid tandem mass spectrometer (Q-QITMS).
  • Selective ion injection using the quadrupole mass filter.

Related Experiment Videos

  • Analysis of perfluorotributylamine (FC-43) and peptide mixtures.
  • Comparison of data obtained with the hybrid system versus QITMS alone.
  • Optimization of ion manipulation within the QITMS.

    • Main Results:

    • The hybrid system demonstrated improved molecular weight, fragmentation, and high-resolution analyses for peptides.
    • A resolution of 33,200 was achieved for angiotensin I.
    • Significant reduction in space charge-induced signal suppression was observed for LSIMS of Glu-fibrinopeptide B.
    • Efficient 'on-the-fly' collision-induced dissociation was performed.
    • Optimal performance was achieved when ions were not rf-isolated in the QITMS.

    Conclusions:

    • The hybrid tandem mass spectrometer offers enhanced analytical performance compared to a standalone ion trap.
    • The instrument effectively reduces space charge effects and improves resolution.
    • Further optimization of data acquisition protocols is needed to address current limitations.