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Quantitative contribution of the collagen network in dentin hybridization
A J Gwinnett1, F R Tay, K M Pang
1Department of Oral Biology and Pathology, School of Dental Medicine, SUNY at Stony Brook 11794-8702, USA.
American Journal of Dentistry
|August 1, 1996
Summary
Dentin hybridization, the resin-reinforced collagen network, does not significantly impact bonded assembly strength. This study examined the micromorphology of the interface with and without collagen present.
Area of Science:
- Dental Materials Science
- Biomaterials Engineering
- Adhesive Dentistry
Background:
- The hybrid zone is crucial for resin-dentin adhesion.
- The role of collagen within the hybrid zone in bond strength is not fully understood.
Purpose of the Study:
- To quantify the contribution of dentin hybridization to bonded assembly strength.
- To visualize the interface micromorphology with and without collagen.
Main Methods:
- Four groups of molar teeth were prepared and bonded with two different adhesive systems (All-Bond 2, Amalgambond).
- Two groups underwent collagenase digestion to remove collagen.
- Bonded assemblies were tested for shear bond strength, and interfaces were examined using SEM and TEM.
Main Results:
- No significant difference in shear bond strength was found between control and collagenase-treated groups.
- SEM and TEM revealed resin penetration into tubules and lateral extensions, but a distinct hybrid zone was absent in collagenase-treated specimens.
- A tight, gap-free junction was observed between resin and dentin without the collagen network.
Conclusions:
- The resin-reinforced collagen network does not significantly contribute to the quantitative strength of dentin bonding for the tested systems.
- Collagen's presence or absence does not alter the overall bond strength, suggesting other factors are more critical.