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Ureaplasma urealyticum-induced urinary tract stones in rats
1Department of Microbiology, School of Medicine, Dokuz Eylül University, Izmir, Turkey.
Abstract:
This study investigated the possible role of Ureaplasma urealyticum, which is predominantly located in the urogenital tract, in the formation of infectious stones. A standardized Ureaplasma urealyticum broth culture isolated from a human urogenital specimen was inoculated into the renal medulla of five male rats (Rattus norvegicus L., Wistar C, weighing 170 +/- 10 g) and the same amount of culture media was used for five identical control rats. Five days after the inoculation, the rats were killed and fresh preparations from the bladders and the inoculated kidneys of both groups were prepared. At the same time biochemical and histopathological analysis of the contents of the bladders and the inoculated kidneys of both groups was performed. Crystal formation within the bladders of the inoculated rats was demonstrated and biochemical analysis of the crystals showed calcium, magnesium and phosphate, which indicated the existence of infection-induced crystals. These findings were absent in the control rats. The role of Ureaplasma in the production of urinary tract infectious stones was thus demonstrated in vivo.
Insights
Ureaplasma urealyticum infection in rats led to the formation of infectious urinary stones containing calcium, magnesium, and phosphate. This study demonstrates the bacteria's role in producing infection-induced urinary tract stones in vivo.
Area of Science:
- Microbiology
- Urology
- Pathology
Background:
- Ureaplasma urealyticum is a common inhabitant of the human urogenital tract.
- The role of Ureaplasma urealyticum in the formation of infectious urinary stones is not fully understood.
Purpose of the Study:
- To investigate the potential role of Ureaplasma urealyticum in the development of infectious urinary stones.
- To demonstrate the formation of infection-induced crystals in vivo.
Main Methods:
- Male Wistar rats were inoculated with Ureaplasma urealyticum in the renal medulla.
- Control rats received sterile culture media.
- Biochemical and histopathological analyses were performed on bladder and kidney samples.
Main Results:
- Crystal formation was observed in the bladders of rats inoculated with Ureaplasma urealyticum.
- Analysis revealed crystals composed of calcium, magnesium, and phosphate.
- No crystal formation was observed in control rats.
Conclusions:
- Ureaplasma urealyticum inoculation induced the formation of infection-related urinary stones in rats.
- The study provides in vivo evidence for the role of Ureaplasma in producing urinary tract infectious stones.