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Isolation and characterization of a cDNA encoding a Translin-like protein, TRAX
1Department of Immunology, National Institute of Health, Shinjuku-ku, Tokyo, Japan.
FEBS Letters
|January 20, 1997
Summary
Researchers identified TRAX, a protein partner for Translin, a DNA-binding protein implicated in lymphoid malignancies. TRAX may facilitate Translin
Area of Science:
- Molecular Biology
- Genetics
- Cancer Research
Background:
- Translin is a DNA-binding protein that recognizes specific sequences at chromosomal translocation breakpoints.
- These translocations are frequently observed in lymphoid malignancies, suggesting Translin's role in these cancers.
Purpose of the Study:
- To investigate the functional significance of Translin at recombination hotspots.
- To identify other proteins that interact with Translin.
Main Methods:
- Yeast two-hybrid system to screen for protein interactions.
- Amino acid homology analysis to characterize identified partners.
Main Results:
- Identified a 33 kDa protein partner for Translin, named TRAX.
- TRAX shares extensive amino acid homology with Translin.
- TRAX possesses bipartite nuclear targeting sequences in its N-terminal region.
Conclusions:
- TRAX interacts with Translin.
- TRAX may be involved in the nuclear transport of Translin, which lacks its own nuclear targeting motifs.