Related Experiment Video
Updated: Aug 12, 2026

Sheathless Capillary Electrophoresis–Mass Spectrometry for Metabolic Profiling of Biological Samples
Published on: October 1, 2016
Use of wide-bore capillaries in constant denaturant capillary electrophoresis
1Division of Toxicology, Massachusetts Institute of Technology, Cambridge 02139, USA.
Abstract:
A serious limitation of capillary electrophoresis in separating mutant from wild-type DNA sequences is the amount of DNA which may be loaded on narrow-bore columns. Because we sought to examine mutant sequences derived from more than 10(9) human cells, we were obliged to overcome this limitation. Thus we examined the feasibility of using wide-bore capillaries of 250, 320 and 540 microns in constant denaturant capillary electrophoresis (CDCE) using conditions which successfully limited the effect of Joule heating. Excellent separations were observed using the wide-bore capillaries comparable to those obtained using a 75 microns capillary. DNA loading capacities were increased almost 100-fold as the bore of capillary increased from 75 to 540 microns.
More Related Videos
10:05Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry
Published on: October 24, 2018
07:54Capillary Electrophoresis Mass Spectrometry Approaches for Characterization of the Protein and Metabolite Corona Acquired by Nanomaterials
Published on: October 27, 2020
Related Concept Videos
Gas Chromatography: Types of Columns and Stationary Phases
For an analyte to remain on the column for a sufficient amount of time, it must exhibit some level of compatibility (or...
High-Performance Liquid Chromatography: Elution Process
Ion-Exchange Chromatography
Electrophoresis: Overview
There...
Capillary Electrophoresis: Instrumentation
Capillary Electrophoresis: Applications
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...