[Stimulation trials of trophoblast cells in vitro using PP14]

Cytotrophoblast cells were isolated from human term placenta after cesarean section by fragmentation of villous tissue with trypsin and DNAse I. Trophoblast cells fuse in vitro to syncytiotrophoblast cells and progesteron is released. Placental Protein 14 (PP14) was incubated (300 micrograms/ml PP14 in 6 ml solution (6 x 10(6) cells) with the cytotrophoblast cells. Production of Progesteron is increased in PP14-trophoblast cell cultures compared to untreated trophoblast cells.