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Lymphocyte-fibroblast interactions

S Murakami1, H Okada

  • 1Department of Periodontology and Endodontology, Osaka University Faculty of Dentistry, Japan.

Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists
|January 1, 1997
PubMed
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It is time to move on.....

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Fibroblasts actively participate in chronic inflammation by interacting with lymphocytes. These cell-cell interactions, mediated by adhesion molecules, influence immune responses and tissue destruction in conditions like periodontitis.

Area of Science:

  • Immunology
  • Cell Biology
  • Pathogenesis of Inflammatory Diseases

Background:

  • Chronic inflammation involves inflammatory cell accumulation and tissue destruction.
  • Lymphocyte interactions with non-hematopoietic cells, like fibroblasts, are crucial in inflammatory sites.
  • Understanding these heterotypic cell-cell interactions is key to clarifying immunopathogenesis.

Purpose of the Study:

  • To elucidate the molecular mechanisms of lymphocyte-fibroblast interactions in chronic inflammatory settings.
  • To investigate the role of fibroblasts as active participants rather than passive bystanders in inflammation.

Main Methods:

  • Investigated lymphocyte-fibroblast binding using various fibroblast cell lines.
  • Employed blocking experiments with monoclonal antibodies against cell adhesion molecules (e.g., VLA, LFA-1/ICAM-1, CD44/hyaluronate).

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  • Assessed the impact of cell adhesion on fibroblast cytokine mRNA expression.

    • Main Results:

    • Activated lymphocytes exhibit strong binding to fibroblasts.
    • Cell adhesion molecules, including VLA integrins, LFA-1/ICAM-1, and CD44/hyaluronate, mediate lymphocyte-fibroblast interactions.
    • Adhesive interactions stimulate fibroblasts to increase inflammatory cytokine mRNA expression.

    Conclusions:

    • Fibroblasts are not passive bystanders but actively contribute to local inflammatory reactions.
    • Lymphocyte-fibroblast interactions play a significant role in lymphocyte lodging, retention, and inflammatory processes.
    • These findings are relevant for understanding chronic inflammatory diseases, such as periodontitis.