Related Experiment Videos
Epidermal growth factor selectively enhances functional enterocyte adaptation after massive small bowel resection
J C Dunn1, C P Parungo, E W Fonkalsrud
1Department of Surgery, Sepulveda Veterans Administration Medical Center, Los Angeles, California, USA.
The Journal of Surgical Research
|January 1, 1997
Summary
Epidermal growth factor (EGF) enhances intestinal adaptation after massive small bowel resection. EGF administration selectively increased sodium-glucose cotransporter 1 (SGLT1) expression, suggesting a therapeutic role for short bowel syndrome.
Area of Science:
- Gastroenterology
- Cell Biology
- Surgical Research
Background:
- Massive small bowel resection triggers intestinal adaptation in enterocytes.
- Enterocyte adaptation involves proliferation and functional changes.
- Selective expression of brush border enzymes and transporters is crucial.
Purpose of the Study:
- To investigate the effect of epidermal growth factor (EGF) on enterocyte functional adaptation after small bowel resection.
- To examine the expression of sucrase, sodium-glucose cotransporter 1 (SGLT1), and NaK ATPase.
- To determine if EGF augments the adaptive response in remnant intestine.
Main Methods:
- Adult Lewis rats underwent ileal transection or 70% proximal intestinal resection.
- Animals received intraperitoneal saline or EGF for 1 week.
- Enterocytes were isolated, and protein expression of sucrase, SGLT1, and NaK ATPase was analyzed via Western blot 4 weeks postoperatively.
Main Results:
- EGF did not alter sucrase, SGLT1, or NaK ATPase expression in transected animals.
- Intestinal resection significantly increased sucrase and SGLT1 expression.
- Combined EGF administration and resection further increased SGLT1 expression.
Conclusions:
- Intraperitoneal EGF selectively enhances SGLT1 expression in enterocytes post-massive small bowel resection.
- EGF augments the adaptive response of the remnant intestine.
- EGF may hold therapeutic potential for managing short bowel syndrome.