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Sporulation of Cyclospora sp. oocysts
H V Smith1, C A Paton, M M Mitambo
1Scottish Parasite Diagnostic Laboratory, Stobhill NHS Trust, Glasgow, United Kingdom. h.v.smith@strath.ac.uk
Applied and Environmental Microbiology
|April 1, 1997
Abstract:
Cyclospora sp. oocysts sporulated maximally at 22 and 30 degrees C for 14 days retarded sporulation. Up to 12% of human- and baboon-derived oocysts previously stored at 4 degrees C for 1 to 2 months sporulated when stored for 6 to 7 days at 30 degrees C.
Insights
Optimal sporulation for Cyclospora oocysts occurred at 22 and 30 degrees C. Refrigerated oocysts showed renewed sporulation after a short incubation at 30 degrees C.
Area of Science:
- Parasitology
- Environmental Health
- Foodborne Pathogens
Background:
- Cyclospora cayetanensis is an important enteric protozoan parasite.
- Understanding oocyst sporulation is crucial for parasite control and transmission studies.
Purpose of the Study:
- To investigate the optimal temperature and duration for Cyclospora sp. oocyst sporulation.
- To determine if refrigerated oocysts can regain sporulation capacity.
Main Methods:
- Incubation of Cyclospora sp. oocysts at various temperatures (22 and 30 degrees C) for 14 days.
- Storage of oocysts at 4 degrees C for 1-2 months, followed by incubation at 30 degrees C for 6-7 days.
Main Results:
- Maximum sporulation was observed at 22 and 30 degrees C within 14 days, with longer incubation times retarding sporulation.
- A significant percentage (up to 12%) of previously refrigerated oocysts resumed sporulation after a short incubation period at 30 degrees C.
Conclusions:
- Temperature significantly influences Cyclospora oocyst sporulation.
- Short-term incubation at 30 degrees C can reactivate sporulation in previously stored Cyclospora oocysts, with implications for diagnostic and research settings.