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Surfaces modified with PEO by the Williamson reaction and their affinity for proteins
L Litauszki1, L Howard, L Salvati
1Abbott Laboratories Diagnostics Division, Department of Materials Chemistry D97D, North Chicago, Illinois 60064-3500, USA.
Journal of Biomedical Materials Research
|April 1, 1997
Summary
Poly(ethylene oxide) (PEO) immobilization on halogenated surfaces reduces protein adsorption. This surface modification effectively minimizes the binding of human serum albumin, immunoglobulin G (IgG), and fibrinogen, showing promise for biomaterial applications.
Area of Science:
- Materials Science
- Biomaterials Engineering
- Surface Chemistry
Background:
- Biomaterial surface properties significantly influence biological interactions, including protein adsorption.
- Controlling protein adsorption is crucial for developing biocompatible materials and preventing adverse biological responses.
Purpose of the Study:
- To immobilize poly(ethylene oxide) (PEO) onto halogenated polymer surfaces using Williamson ether synthesis.
- To evaluate the impact of PEO surface modification on the adsorption of key proteins from various biological fluids.
Main Methods:
- Williamson ether synthesis was employed to graft PEO onto halogenated poly(vinylidene chloride), polypropylene, and poly(ethylene terephthalate) surfaces.
- Surface characterization was performed using X-ray photon spectroscopy and water contact-angle measurements.
- Protein adsorption studies were conducted using human serum albumin, immunoglobulin G (IgG), fibrinogen, and murine IgG in phosphate buffer and pooled human sera.
Main Results:
- Successful immobilization of PEO onto the tested halogenated surfaces was confirmed by surface analysis techniques.
- A significant reduction in the adsorption of human serum albumin, IgG, and fibrinogen was observed on PEO-modified surfaces.
- The degree of protein adsorption reduction was dependent on the initial protein concentration in the solution.
Conclusions:
- PEO immobilization via Williamson ether synthesis is an effective strategy for creating protein-repellent surfaces.
- This surface modification approach holds potential for enhancing the biocompatibility of various polymeric materials.
- The findings suggest that PEO-grafted surfaces can mitigate non-specific protein adsorption, which is beneficial for biomedical device applications.