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Related Experiment Videos

Positive interference in lithium determinations from clot activator in collection container

M Sampson1, M Ruddel, S Albright

  • 1Clinical Pathology Department, Warren G. Magnuson Clinical Center, National Institutes of Health, Bethesda, MD 20892, USA. msampson@nih.gov

Clinical Chemistry
|April 1, 1997
PubMed
Summary

Blood collection tubes containing silica clot activators or silicone surfactants can interfere with lithium testing. This interference affects ion-selective electrode accuracy and is not detected by standard control materials.

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Area of Science:

  • Clinical Chemistry
  • Analytical Chemistry
  • Biomedical Engineering

Background:

  • Accurate lithium (Li) determination is crucial for therapeutic drug monitoring.
  • Ion-selective electrode (ISE) methods are commonly used for Li measurement.
  • Potential interferences in blood collection tubes can impact assay results.

Purpose of the Study:

  • To investigate positive interference in lithium ISE determination.
  • To identify the source of interference in specific blood collection tubes.
  • To assess the impact of tube formulation and blood volume on Li measurement.

Main Methods:

  • Evaluation of lithium ISE measurements using Lytening 2Z analyzer.
  • Comparison of blood collected in original and modified Vacutainer Plus Tubes.

Related Experiment Videos

  • Assessment of interference related to silica clot activator and silicone surfactant.
  • Analysis of interference variability with different blood volumes.

    • Main Results:

    • Positive interference observed with lithium ISE determination when using specific Vacutainer Plus Tubes.
    • Interference attributed to silica clot activator or silicone surfactant.
    • Interference inversely correlated with blood volume in the collection tube.
    • Long-term exposure to interfering substances led to decreased Li values and undetected interference in patient samples.

    Conclusions:

    • Silica clot activators and silicone surfactants in blood collection tubes can cause significant positive interference in lithium ISE testing.
    • The observed interference is dependent on the tube formulation and blood volume.
    • Standard control materials may not detect this type of interference, posing a risk for accurate patient Li level assessment.