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Do specific linoleate 13-lipoxygenases initiate beta-oxidation?
I Feussner1, H Kühn, C Wasternack
1Institut für Pflanzenbiochemie, Halle, Germany. ifeussne@ipb.uni-halle.de
FEBS Letters
|April 7, 1997
Summary
Oilseed plants mobilize storage lipids for seedling growth using linoleate 13-lipoxygenase. This enzyme oxygenates fatty acids, suggesting 13(S)-hydro(pero)xy-9(Z),11(E)-octadecanoic acid is key for beta-oxidation during germination.
Area of Science:
- Plant Physiology
- Biochemistry
- Molecular Biology
Background:
- Lipid mobilization is crucial for oilseed plant germination, providing the primary carbon source.
- The precise mechanisms governing lipid mobilization remain largely unelucidated.
Purpose of the Study:
- To investigate the role of linoleate 13-lipoxygenase in the lipid mobilization process during oilseed plant germination.
- To explore the potential involvement of oxygenated fatty acids in beta-oxidation.
Main Methods:
- Monitoring linoleate 13-lipoxygenase induction and translocation to lipid bodies.
- Analyzing the occurrence of hydro(pero)xy polyenoic fatty acid derivatives in storage lipids.
- Comparing the cleavage rates of oxygenated versus non-oxygenated fatty acids.
Main Results:
- Linoleate 13-lipoxygenase is induced and targets lipid bodies during germination.
- Enantiospecific hydro(pero)xy fatty acid derivatives are found in storage lipids, indicating enzyme activity.
- Oxygenated fatty acids, specifically 13(S)-hydro(pero)xy-9(Z),11(E)-octadecanoic acid, are preferentially cleaved.
Conclusions:
- Linoleate 13-lipoxygenase plays a significant role in oilseed germination.
- 13(S)-hydro(pero)xy-9(Z),11(E)-octadecanoic acid is likely the endogenous substrate for beta-oxidation during lipid mobilization.