Use of west-western blotting for detection of adenovirus receptors

A modification of an immunoblotting procedure was developed to detect virus receptors: a nitrocellulose membrane containing electrotransferred cellular proteins separated by polyacrylamide gel electrophoresis in the presence of SDS is treated with the whole virus (viral proteins) and an antiserum against individual viral proteins, followed by the detection of the receptors after their incubation with peroxidase labeled anti-species antibodies by enhanced chemiluminescence. The method detects adenovirus receptors for the capsid proteins including 20- and 80-kD receptors for the fiber, 20- and 40-kD receptors for the penton, and 80- and 200-210-kD receptors for protein IIIa.