Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

PcG complexes and chromatin silencing

V Pirrotta1

  • 1Department of Zoology, University of Geneva, 30 quai Ernest Ansermet, CH1211, Geneva, Switzerland. pirrotta@sc2a.unige.ch

Current Opinion in Genetics & Development
|April 1, 1997
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Polycomb and trithorax control genome expression by determining the alternative epigenetic states of chromatin for key developmental regulators].

Genetika·2011
Same author

Polycomb silencing mechanisms and genomic programming.

Ernst Schering Research Foundation workshop·2006
Same author

Functional dissection of the Suppressor of UnderReplication protein of Drosophila melanogaster: identification of domains influencing chromosome binding and DNA replication.

Genetica·2005
Same author

Polycomb silencing mechanisms in Drosophila.

Cold Spring Harbor symposia on quantitative biology·2005
Same author

Influence of the SuUR gene on intercalary heterochromatin in Drosophila melanogaster polytene chromosomes.

Chromosoma·2003
Same author

The Drosophila suppressor of underreplication protein binds to late-replicating regions of polytene chromosomes.

Genetics·2002

Repressive silencing complexes in yeast and flies share similarities in assembly and function. Their stability and extent depend on component concentration and interactions, influencing chromatin structure and antagonizing transcription.

Area of Science:

  • Molecular Biology
  • Genetics
  • Epigenetics

Background:

  • Silencing complexes are crucial for gene regulation in eukaryotes.
  • Similarities observed between silencing complexes in yeast and Drosophila melanogaster suggest conserved mechanisms.

Purpose of the Study:

  • To elucidate the assembly, stability, and functional mechanisms of eukaryotic silencing complexes.
  • To understand how these complexes interact with chromatin and antagonize transcriptional activity.

Main Methods:

  • Comparative analysis of silencing complex components and functions in yeast and fly models.
  • Investigation of the role of component concentration and interactions in complex assembly and stability.
  • Examination of the interplay between silencing complexes and transcriptional machinery.

Related Experiment Videos

Main Results:

  • Silencing complexes are assembled via a recruitment chain, with stability influenced by component concentration.
  • These complexes affect extended chromatin regions, likely through nucleosome interactions.
  • Transcriptional activity antagonizes complex assembly, while interactions with other complexes favor it.

Conclusions:

  • Silencing complexes represent a conserved mechanism for gene repression across eukaryotes.
  • These complexes likely interfere with transcription by competing with activator binding sites on chromatin.